Isotherm® SARS-CoV-2-RNA-screen

Unlike other commercially available systems based on the principle of polymerase chain reaction with preliminary preparation of samples with kits for the isolation of nucleic acids and a reverse transcription stage, in the test system “Isotherm SARS-CoV-2 RNA-screen”, developed by the group of companies “Medico- Biological Union" and the registered company "GENERIUM", the method of loop isothermal amplification was used.

This significantly reduces the sample analysis time (by more than 3 times) and reduces the possibility of sample mix-up by reducing the number of pre-preparation stages. In this system, it is enough to simply warm up the samples in the lysis buffer, transfer them to the plate, add the reaction mixture, and carry out amplification. Store the kit at temperatures from +2 to +8 degrees Celsius.

Attention!

A hotline has been set up for questions regarding testing.

Phone No.: 8 (495) 988-47-94

on technical characteristics and methodological features of the test system – dial ext. 7105

regarding the purchase of a test system – ext. 7060

on the diagnostic significance of the test system – ext. 7098

e-mail: support.testsystem@generium.ru

Method

To determine the presence or absence of infectious agents in human biological fluids, amplification of the genetic material of the infectious agent is carried out. The test system uses loop isothermal amplification. This technique is based on the reaction of nucleic acid synthesis with strand displacement, catalyzed by DNA polymerase I from the thermophilic bacterium Bacillus stearothermophilus. Distinctive features of loop isothermal amplification are the reaction at a constant temperature of 60–65 °C, rapid accumulation of reaction products (15–60 min), the ability to combine the reaction with reverse transcription in one tube, low sensitivity to impurities, and the ability to use blood as a starting matrix or other biological fluids. Detection of the presence or absence of SARS-CoV-2 coronavirus RNA in human biological fluids includes two stages: extraction of SARS-CoV-2 coronavirus RNA from analyzed samples and loop isothermal amplification using specific oligonucleotide sequences and modified DNA polymerase I from thermophilic bacteria Bacillus stearothermophilus.

Final result speed

Pre-processing of samples will take 35 minutes, amplification time – 25 minutes. Thus, the result for one sample can be obtained in 1 hour.